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KMID : 0379119990270030181
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Korean Journal of Mycology
1999 Volume.27 No. 3 p.181 ~ p.186
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Identification and Characterization of Osmotolerant Yeast Isolated from Soy Paste
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구본성/Koo, Bon Sung
류진창/이두형/이승범/변명옥/송재경/Ryu, Jin Chang/Lee, Du Hyung/Lee, Seung Bum/Byun, Myung Ok/Song, Jae Kyeong
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Abstract
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Osmotolerant yeast isolated from soy paste could grow on media with 2 M NaCl. This strain was identified as Zygosaccharomyces rouxii by biological characteristics, RFLP of ribosomal DNA and mating with compatible haploid strain. Growing rate of the Z. rouxii YDJ was slower than Saccharomyces cerevisiae. Z. rouxii YDJ accumulated trehalose, which is known as one of the osmolytic protectants, in cells cultured on media with salt. Enzyme activity of trehalose phosphate synthase related to trehalose biosynthesis of the YDJ was lower than those of S. cerevisiae. Trehalase activity related trehalose degradation was also lower in Z. rouxii YDJ than S. cerevisiae. However, as Z. rouxii accumulated trehalose by salt treatment, salt tolerancy of Z. rouxii was assumed to be related to trehalose in addition to glycerol.
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